Search results for "fluorescent labeling"

showing 3 items of 3 documents

Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in …

2019

Some strains of lactic acid bacteria (LAB) produce riboflavin, a water-soluble vitamin of the B complex, essential for human beings. Here, we have evaluated riboflavin (B2 vitamin) production by five Lactobacillus plantarum strains isolated from chicha, a traditional maize-based fermented alcoholic beverage from north-western Argentina and their isogenic riboflavin-overproducing derivatives previously selected using roseoflavin. A direct fluorescence spectroscopic detection method to quantify riboflavin production in bacterial culture supernatants has been tested. Comparison of the efficiency for riboflavin fluorescence quantification with and without prior HPLC fractionation showed that th…

Microbiology (medical)Otras Ingenierías y Tecnologíaslcsh:QR1-502RiboflavinINGENIERÍAS Y TECNOLOGÍASBacterial growthMicrobiologyRIBOFLAVINlcsh:Microbiologylaw.inventionAlimentos y Bebidas03 medical and health sciencesProbioticlawIn vivoLACTIC ACID BACTERIAriboflavin//purl.org/becyt/ford/2.11 [https]Original Research030304 developmental biology0303 health sciencesbiology030306 microbiologyChemistrydigestive oral and skin physiologyPROBIOTICfluorescent labelingfood and beveragesLACTOBACILLUS PLANTARUMbiology.organism_classificationlactic acid bacteriaB vitamins//purl.org/becyt/ford/2 [https]BiochemistrymCherryFLUORESCENT LABELINGprobioticBacteriaLactobacillus plantarumLactobacillus plantarum
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In vivo analysis of noise dependent activation of white blood cells and microvascular dysfunction in mice

2021

This article contains supporting information on data collection for the research article entitled “Aircraft noise exposure drives the activation of white blood cells and induces microvascular dysfunction in mice” by Eckrich et al. We found that noise-induced stress triggered microvascular dysfunction via involvement of innate immune-derived reactive oxygen species. In this article, we present the instrumentation of mice with dorsal skinfold chambers for in vivo microscopic imaging of blood flow, interaction of leukocytes with the vascular wall (also by fluorescent labelling of blood cells) and vessel diameter. In addition, we explain the preparation of cerebral arterioles for measurement of…

chemistry.chemical_classificationReactive oxygen speciesPathologymedicine.medical_specialtyScienceQClinical BiochemistryIn vivo analysisVideo microscopyBlood flowMethod ArticleIn vivo fluorescence microscopy and cerebral arteriole cannulation to assess noise induced changes in activation of white blood cells and microvascular dysfunctionIn vitroCerebral arterioles cannulationMedical Laboratory TechnologyDorsal skinfold chamberchemistryIn vivoFluorescent labeling of blood cellsVideo microscopyMicroscopic imagingmedicineResearch articleMethodsX
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The effect of PLC-inhibitor on echovirus 1 internalization probed with fluorescently labeled echovirus 1

2014

Echovirus 1 (EV1) kuuluu Pikornavirusperheeseen ja tarkemmin luokiteltuna Enterovirusten sukuun. EV1 on pieni ja vaipaton RNA-virus, jonka proteiinikuori koostuu neljästä proteiinista VP1-VP4. Infektion on osoitettu alkavan, kun EV1 tarttuu α2β1-integriiniin solun pinnalla, jonka jälkeen virus otetaan solun sisään kaveoliinivälitteistä endosytoosia sekä makropinosytoosia muistuttavalla mekanismilla. Toisaalta sitä, kuinka virus avautuu sekä vapauttaa RNA:nsa, ei vielä tarkalleen tiedetä. Tässä tutkimuksessa EV1 puhdistettiin ensin sakkaroosigradientilla, jonka jälkeen virus leimattiin fluoresoivalla amiinireaktiivisella värillä. Tulokset osoittivat, että leimauksen onnistumiseksi tarvitaan …

viruksetfluoresenssifluorescent labelingECHO-viruksetEchovirus 1uncoatingendocytosis inhibitorendosytoosiinhibiittorit
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